Path of Exile 3.27 introduces one of the most exciting endgame systems to date - the Bloodline Ascendancies. Also called "Boss Ascendancies," these new additions bring a fresh layer of customization, allowing players to combine their traditional Ascendancy with a powerful new specialization obtained by defeating unique bosses. Whether you're a veteran mapper or diving into your first Eternal Labyrinth, understanding how Bloodline Ascendancies work is essential for maximizing your build in PoE 3.27.

For players gearing up their characters or preparing for endgame grinds, it's more important than ever to manage your Path of Exile Items efficiently - especially as Bloodline challenges demand both preparation and optimization.

What Are Bloodline Ascendancies?

Added in Path of Exile 3.27, Bloodline Ascendancies expand the traditional Ascendancy system. Every character can now combine one classic Ascendancy (like Trickster, Slayer, or Necromancer) with one Bloodline Ascendancy (such as Animist Farrul or Herald Lycia).

After completing the Eternal Labyrinth, you'll unlock eight shared Ascendancy points, which can be distributed between both your Ascendancies. Typically, this lets you allocate around four Bloodline notables, giving you access to new hybrid effects and unique synergies.

Note: The Scion's Ascendant class works differently, with its own special interactions between classic and Bloodline Ascendancies.

Since this system was introduced in patch 3.27, some details remain subject to change as Grinding Gear Games finalizes balance and boss behavior.

How to Unlock and Respec Bloodline Ascendancies

To unlock a Bloodline Ascendancy, you'll need to defeat the associated boss tied to that Bloodline. Once defeated, the option becomes available for allocation in your Ascendancy tree.

Respeccing Bloodline points works exactly like normal Ascendancies:

·5 Respec Points from quests, or

·5 Regret Orbs, or

·Paying a gold fee to Faustus.

Pro Tip: Before level 83, Faustus' respec fee is manageable. However, above level 90, his gold costs become absurdly high - use Regret Orbs instead to save currency.

Full List of Bloodline Ascendancies

Each Bloodline is tied to a specific boss encounter. Some are easier to obtain than others, and a few are deep-endgame grinds reserved for the most dedicated Exiles.

1. Animist Farrul

·Boss: Farrul, First of the Plains

·How to Unlock: Encounter a Farric Tiger Alpha in an Einhar mission, capture it, then sacrifice it at Einhar's Menagerie.

Farrul's Bloodline is one of the most accessible and beginner-friendly. It enhances hybrid minion and melee/spell builds, rewarding aggressive playstyles that stay close to the action.

2. Herald Lycia

·Boss: Lycia, Herald of the Forbidden Sanctum

·How to Unlock: Found on the deepest floors of the Forbidden Sanctum, accessed through Forbidden Tomes dropped by map monsters.

Lycia's Bloodline is a top choice for Herald and Elemental builds. It grants access to a unique Herald notable, elemental conversion mechanics, and profane ground interactions - making it a powerful option for Inquisitors, Elementalists, or Hex-based casters.

3. Delirious Imposter (Tangmazu)

·Boss: Tangmazu, the Delirious Imposter

·How to Unlock: Complete the Simulacrum, the ultimate Delirium encounter.

This Bloodline provides extreme offensive or defensive power, but every node comes at a steep tradeoff. Tangmazu's tree is ideal for advanced players who want to min-max damage or mitigation at the cost of convenience or safety.

4. Lich Catarina

·Boss: Catarina, Master of Undeath

·How to Unlock: Defeat her in her Syndicate Hideout, accessed via Syndicate Medallions dropped from Betrayal safehouses.

Catarina's Bloodline is perfect for minion-focused builds - especially those that rely on self-casting minions or hybrid summoner setups. Her nodes grant bonuses to spell damage, minion health, and corpse-based mechanics, blending offense and utility in a true necromantic style.

5. Primordial Knight Aul

·Boss: Aul, the Crystal King

·How to Unlock: Found deep within the Delve Mines. Aul appears rarely beyond depth 200, and more consistently after depth 500.

Aul's Bloodline gives all classes a taste of Juggernaut or Trickster-like resilience, offering action speed reduction immunity and elemental mitigation. While weaker than class-exclusive defenses, it's a valuable option for glass-cannon builds looking for extra toughness.

6. Runesmith Olroth

·Boss: Olroth, Origin of the Fall

·How to Unlock: Encountered in level 81+ Expedition Logbooks, specifically when investigating Knights of the Sun sites.

Olroth's Bloodline introduces powerful Ward-scaling mechanics. It's ideal for hybrid Life/Ward setups or characters experimenting with innovative defensive layers - particularly when you buy POE Items that enhance Ward interactions.

7. King in the Mists

·Boss: King in the Mists

·How to Unlock: Use an Audience with the King, found occasionally in Ritual reward windows.

This is a highly experimental and niche Bloodline. It allows bizarre and powerful interactions - often enabling mechanics that were previously impossible - but always comes with drawbacks. Expect to trade consistency for creativity here.

8. Oshabi, Avatar of the Grove

·Boss: Oshabi

·How to Unlock: Use a Sacred Blossom in the Map Device (drops from Harvest bosses).

Oshabi's Bloodline taps into Azmeri nature magic, offering strong synergy with life, growth, and regeneration-based mechanics. It's flexible enough to support melee, caster, or summoner setups that benefit from sustained combat and scaling regeneration.

9. The Trialmaster

·Boss: The Trialmaster

·How to Unlock: Rarely appears in Ultimatum encounters (Tier 14+), or guaranteed via Ultimatum Scarab of Dueling.

Trialmaster's Bloodline specializes in Vaal skills and corrupted item synergies. This makes it one of the most unique Bloodlines in Path of Exile, perfectly suited for players who embrace chaos and risk for massive power spikes.

Final Thoughts

Path of Exile 3.27's Bloodline Ascendancies are a massive leap forward in customization. By combining traditional and Bloodline Ascendancies, players can create hybrid builds that were never possible before - such as minion-based melee Juggernauts or self-healing Elemental Tricksters. And these new boss-based paths promise to redefine endgame build diversity in PoE 3.27 and beyond.